物質の持つ特定波長の光を吸収する性質を利用した検出器。次のようなものが存在している。
ディテクターから出力された、電気信号を記録し、そこからピークを検出、解釈を行う。結果は、感熱紙等に印字される。装置のコントロールをしないのであれば、どのメーカーの物を使用しても問題はないが、通常は、装置のコントロールも同時に行うため、同じメーカーの物を選択する。
ポンプの押し出す部分が一つのポンプ。古典的システムにおいては標準的な仕様であったが、現在は移動相脈動を軽減させるためやグラジェント分析が主流となりつつあるため、主たる移動相の送液のために用いられることは少なく、蛍光検出器のための標識試薬を送液するために用いられることが多い。但し、高い精度を要求しない分析ではこの仕様で十分事足りる、機器の価格が安い、メンテナンスが容易等の利点もあるため現在でも使用されている。
The Examination is challenging through the intricate matrix of serum samples. A solid-section extraction followed by an HPLC analysis using a fluorescence detector provides the mandatory selectivity and detection restrictions.
Allow or Disable Cookies In case you disable this cookie, we won't be in a position to preserve your Choices. Which means anytime you take a look at this website you need to empower or disable cookies all over again.
분석물의 피크 면적 값(=검출기의 응답)은 정량화를 위해 사용됩니다. 분석자는 분석을 수행하기 전, 분석물의 표준 용액(기지 농도의 시액)을 몇 가지 측정하고, 시료 농도와 획득한 피크 면적 값에 의해 도표된 검량선을 그립니다.
규제 약물(마약, 합성 마약, 대마, 각성제, 향정신성 의약품, 아편양제제 등), 반도핑 관련(금지 물질, 금지 약물, 스테로이드 등), 약물 대사물
. A person issues with an isocratic elution is always that an proper mobile period toughness for resolving early-eluting solutes could cause unacceptably lengthy retention periods for late-eluting solutes. Optimizing the cellular phase for late-eluting solutes, Then again, could deliver an inadequate separation of early-eluting solutes.
스포츠 도핑에서 약물 검사까지 법의독성학 응용 분야에 적용되는 방법에 대해 알아보세요.
The preferred HPLC detectors take full advantage of an analyte’s UV/Vis absorption spectrum. These detectors vary from very simple layouts, during which the analytical wavelength is chosen employing here suitable filters, to the modified spectrophotometer during which the sample compartment features a flow cell.
*본 포스팅의 저작권은 써모 피셔 사이언티픽에 있으며, 콘텐츠의 무단 복제 및 수정, 재배포를 금지합니다.
If the answer is diluted the area of the peak will probably be considerably less, when the detention time might be identical. So it is possible to detect a substance present even in an incredibly smaller amount.
The sample injector introduces the sample read more into the HPLC system. Exact and accurate sample injection is important for getting dependable effects.
The injector introduces a precise quantity of the sample Remedy in to the mobile phase stream. Several injection techniques exist, with loop injection becoming a standard system.